TIGR Closure Tools
|Scientific software for the assembly/finishing stage of DNA sequencing projects.|
This is software for the whole-genome shotgun method of sequencing DNA: randomly selected fragments of sheared DNA are cloned in libraries characterized by fragment size; both ends of each fragment are sequenced by Sanger chemistry with an instrument such as the ABI 3730; fragment reads are computationally re-assembled with software such as the Celera Assembler; gaps in the assembly are closed by manual inspection, directed re-sequencing, and regional re-assembly; finished sequence is fed to software or teams of experts for annotation that reveals the encoded gene content. This software was developed at TIGR where it contributed to the sequencing and publication of hundreds of microbial genomes.
This package of tools includes...
This software was developed at The Institute for Genomic Research (TIGR) with financial assistance from the Microbial Sequencing Centers (MSC), part of the National Institute of Allergy and Infectious Disease (NIAID) at the National Insitutes of Health (NIH). The source is hosted by SourceForge.